2016中国农业科学院作物所作物育种专业博士入学考试真题

2016中国农业科学院作物科学研究所作物育种学博士入学考试试题（回忆版）

1分子遗传学（共100分）

1.1名字解释

朊病毒；宏基因组学；基因组印迹；组蛋白修饰；染色质重塑；表观遗传修饰；沉默子与绝缘子；程序性细胞死亡；同源重组；RNA干扰

1.2简答题

简述至少3种基因克隆技术

列举至少3种验证DNA与蛋白互作的技术

简述DNA损伤修复的方式

1.3论述题（3选2）

论述基因编辑技术CRISPR/Cas9的技术原理和特点及应用

论述第一代、第二代和第三代测序技术的特点

列举2015年分子遗传学领域重大研究进展（国内国外各5例）

2作物育种学（共100分）

2.1简答题

高产作物源、库、流的特征

简述引种的原理及影响因素

简述染色体结构变异的几种方式及遗传学效应

简述杂种优势的原理及复杂多样性特征

简述表型与基因型鉴定的几种方式

2.2论述题

如何制定育种策略体现绿色环保的概念

发掘创制种质资源及提高利用效率的方法

论述分子育种的基本策略

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