HPLC法测定前列康复胶囊中大车前苷、朝藿定C和淫羊藿苷

HPLC法测定前列康复胶囊中大车前苷、朝藿定C和淫羊

藿苷

李征;罗晶;潘延宁

【期刊名称】《现代药物与临床》

【年(卷),期】2015(000)008

【摘要】Objective To establish a method for simultaneous determination of plantamajoside, epimedin C, and icariin in Qianlie Kangfu Capsules. Methods The determination was carried out on Agilent Zorbax C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile - 0.1% phosphoric acid solution with gradient elution at a flow rate of 1.0 ml/min. The column temperature was set at 35 ℃ and the detection wavelengths were 330 nm in 0—30 min (determination of plantamajoside) and 270 nm in 30—50 min (determination of epimedin C and icariin). Results Plantamajoside, epimedin C, and icariin had good linearity in the ranges of 10.08 —201.60 ng, 18.43 —368.60 ng, and 52.25 —1 045.00 ng, respectively. The average recoveries were 100.6%, 97.2%, and 97.2%with RSD 1.5%, 1.3%, and 1.2%, respectively. Conclusion The method is simple and repeatable, which can be applied to the quality control for Qianlie Kangfu Capsules.%目的：建立同时测定前列康复胶囊中大车前苷、朝藿定C、淫羊藿苷的HPLC法。方法安捷伦Zorbax C18色谱柱（250 mm×4.6 mm，5μm）；以乙腈–0.1%磷酸水溶液为流动相，梯度洗脱；柱温35℃；检测波长