3种芹菜茎组织总RNA提取方法的比较研究

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3种芹菜茎组织总RNA提取方法的比较研究

作者：董姻然陈湘宁常希光等

来源：《安徽农业科学》2014年第08期

摘要 [目的]为了获得质量较好的芹菜茎的总RNA，为后续分子生物学试验提供基础。[方法]试验对1种RNA提取方法（Trizol法）和2种RNA提取试剂盒（RNA prep Pure Plant Kit 法、RNA simple Total RNA Kit法）方法进行比较研究。[结果]RNA prepPure Plant Kit方法得

率低，且OD260/OD280值低于1.8，Trizol法OD260/OD230高于2.0，且得率低于RNA simple Total RNA kit法。只有RNA simple Total RNA kit法提取的RNA质量最好，得率最高。[结论]与Trizol法和RNA prep Pure Plant Kit法相比，RNA simple Total RNA Kit法提取的芹菜茎总RNA得率高，完整性好，凝胶电泳泳道无杂质，可以满足半定量RTPCR的试验需要。

关键词芹菜；总RNA提取；RNA simple Total RNA Kit法

中图分类号 S636.3 文献标识码 A 文章编号 0517-6611（2014）08-02273-03

The Comparison of Several Kinds of Methods for Extracting Total RNA from the Stalk of Celery

DONG Yinran， CHEN Xiangning et al

（Department of Food Science and Engineering， Beijing University of Agriculture， Beijing 102206； Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue， Beijing 102206）

Abstract [Objective] In order to obtain better quality total RNA in the stalk of celery and lay the foundation for subsequent molecular biology experiments. [Method] One RNA extraction method （Trizol method） and two RNA extraction kits （RNA prep Pure Plant Kit method， RNA simple Total RNA Kit method） were compared. [Result] The yield of RNA prepPure Plant Kit is low，

OD260/OD280 value is lower than 1.8， OD260/OD230 of Trizol method is higher than 2.0， the yield is lower than RNA simple Total RNA kit method. RNA extracted by RNA simple Total RNA kit has the best quality and the highest yield. [Conclusion] The results showed that the Trizol method was better than two RNA extraction kit method， the total RNA isolated with Trizol method had high integrity， high yield， good stability， and no pollution in the gel electrophoresis track. And then could meet the needs of the semiquantitative RTPCR test.

Key words Celery； Total RNA extraction； RNA simple Total RNA Kit method