Impact of blood pressure control on coronary flow reserve in hypertensive patients

tively guide catheter ablation of persistent AF．

（Authors）

2016310Ｒole and related mechanism of S1P/S1P1 signal pathway during post conditioning of hyper-trophic cardiomyocytes．BAO Xinhui（包馨慧），et al．Heart Center，1st Affil Hosp，Xinjiang Med Univ，Uru-mqi830054．Chin J Cardiol2016；44（5）：431-435．Objective To study the role and mechanism of sphingosine-phosphate（S1P）/sphingosine-1-phosphate receptor1（S1P1）signal pathway during post condition-ing of hypertrophic cardiomyocytes．Methods Neonatal rat cardiomyocytes were isolated and cultured，then stim-ulated by norepinephrine（NE）to induce cardiomyocytes hypertrophy．Using tri-gas incubator to create hypoxia and reoxygenation enviroment to mimic ischemia-reperfu-sion and postconditioning．Hypertrophic cardiomyoctyes were divided into five groups according to the presence or absence of various drugs and postconditiong and rele-vant signal pathways changes were detected：（1）IPost group（hypoxia+postconditioning）；（2）IPost+S1P group（cells were pretreated with S1P（1μmol/L）for2 h before IPost）；（3）IPost+W-146+S1P group（cells in IPost+W-146+S1P group were pretreated with S1P1inhibitor W-146（0．4μmol/L）for20min）；（4）IPost+PD98059+S1P group（cells in IPost+S1P group were pretreated with MAPK antagonist PD98059（125μmol/L）for20min；（5）IPost+LY-294002+ S1P group（cells in IPost+S1P group were pretreated with PI3K antagonist LY294002（0．1μmol/L）for20 min）．Apoptosis was detected by flow cytometry and protein expression of relevant signal pathways were de-tected by Western blot．Ｒesults（1）Apoptosis rate was significantly increased in hypoxia/reoxygenation（27．90?4．49）%group compared wtih normal control group （7．97?2．18）%，which could be significantly reduced in IPost group（15．90?1．77）%（all P＜0．05）．（2）Apoptosis rate and caspase-3expression were both significantly lower in IPost+S1P and IPost+S1P+LY-294002groups than in IPost and IPost+S1P+W-146 and IPost+S1P+PD98059group（all P＜0．05）．（3）p-EＲK1/2expression was significantly higher in IPost+S1P and IPost+S1P+LY-294002group than in IPost and IPost+S1P+W-146group and IPost+S1P +PD98059group（all P＜0．05）while p-Akt expres-sion was similar among IPost，IPost+S1P+W-146and IPost+S1p+PD98059group．p-EＲK1/2and p-Akt levels in IPost+S1P+W-146group and IPost+S1P+ PD98059were similar as in IPost group．Conclusion S1P can play protective role in NE induced cardiomyo-cytes hypertrophy during post conditioning through downregulating caspase-3expression and reducing apop-tosis rate via targeting S1P1and activating EＲK1/2sig-nal pathway．

（Authors）

2016311Impact of blood pressure control on coro-nary flow reserve in hypertensive patients．DU Lan-fang（杜兰芳），et al．Dept Cardiol，Peking Univ，3rd Hosp，Key Lab Cardiovasc Mole Biol＆Ｒegul Dept，Health Ministry，Beijing100191．Chin J Cardiol2016；44（5）：421-425．

Objective To investigate the impacts of blood pres-sure control on coronary flow reserve（CFＲ）in hyperten-sive patients．Methods A total of236patients without significant coronary stenosis（defined as＜50%luminal narrowing confirmed by coronary angiography or coronary artery CT scan）between January2011and July2015 were retrospectively enrolled in this study．CFＲwas measured in the left anterior descending coronary artery （LAD）during adenosine triphosphate-induced hypere-mia by transthoracic Doppler echocardiography．Patients were divided into hypertension group（n=173）and non-hypertension group（n=63）．The hypertension patients were further divided into ideally controlled（n=31，de-fined as SBP＜120mmHg（1mmHg=0．133kPa）and DBP＜80mmHg），controlled（n=82，defined as SBP 120to139mmHg and DBP＜90mmHg）and uncon-trolled groups（n=60，defined as SBP≥140mmHg and /or diastolic DBP≥90mmHg）based on their blood pressure after systematic antihypertensive therapy and CFＲvalues were compared among the4groups．Multiva-riate regression analyses were performed to identify the independent determinants of CFＲin patients with hyper-tension．Ｒesults Compared with non-hypertension group，the CFＲwas significantly lower in controlled （3．27?0．71vs．2．87?0．56，P＜0．001）and uncon-

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·China Medical Abstracts（Internal Medicine）