3D Deconvolution Look Ahead to Get Ahead

All parameters needed for deconvolution are automatically read from an AxioVision Z-stack.Documentation in new dimensions The 3D Deconvolution from Carl Zeiss contains everything you can expect from advanced digital imaging today.

? Z-stack – Reproducibly controlled with nanometer precision

? Multichannel – Stored in up to eight independent channels

?Time-series – Dynamic processes of live specimens recorded in "movie" fashion

? Mark&Find – Retrieve the correct position -quickly and precisely

And here’s another essential consideration in your daily research work: All functions can be learned and mastered in a very short time.

BEFORE AFTER

Benefits in theory and practice

The more exactly the degree of distortion and, thus, the point spread function (PSF) is known, the more completely can convolution be undone. 3D Deconvolution fr om Car l Zeiss with its "theor etical PSF" offer s a method with solid advantages: outstanding r esults, low time consumption, and little defining effort. And what is more: All parameters are automatically stored with the images for automatic readout at any time. A deconvolution once stored can be used again even year s later – without the r isk of inaccurate parameter inputs.

Deconvolution

Leading-edge technologies

Optimized for su preme image qu ality and

performance, the algorithms of the 3D

Deconvolution software are the latest results

o f i n t e n s i v e re s e a rc h a n d e n g i n e e r i n g.

Carl Zeiss 3D Deconvolution offers you three

methods perfectly tailored to your requirements.

1. Nearest Neighbor

A 2D method applied to the entire 3D stack –

the method of choice where results are needed

within seconds.

2. Inverse Filter – regularized

A genu ine 3D calcu lation method, ideal for

calcu lating several image stacks and for

preselecting images. This method determines

the calculation parameters automatically and

delivers good results within a short time.

3. Iterative Maximum Likel ihood

algorithm

The sophisticated method for the ultimate in

imaging qu ality. The only method permitting

the calculated image stack to be sliced in any

plane and visualized in 3D with mathematical

precision.

BEFORE AFTER

BEFORE AFTER

Property tabs for defining the point spread function and handling the deconvolution process.

Carl Zeiss

Light Microscopy

P.O.B. 4041

37030 G?ttingen GERMANY

Phone: ++49 551 5060 660Telefax: ++49 551 5060 464

Internet: www.zeiss.de/axiovision d o n e n v i r o n m e n t -f r i e n d l y p a p e r ,h e d w i t h o u t t h e u s e o f c h l o r i n e .c t t o c h a n g e .

40-715e /03.2001Microscopes

Axiovert 200 M, Axioplan 2 imaging MOT, Axioskop 2 MOT, or the laser scanning systems LSM 510 and LSM 5 P ASCAL. The motorized high-end microscopes from Carl Zeiss excel on all counts:superior optics, optimized fluorescence, perfect ergonomic design, unsurpassed flexibility, and intelligent details such as the patented Light Trap.

Camera

Optimum integration with the microscope's and user software, finest resolution and highest sensitivity: the Carl Zeiss AxioCam family,monochrome version.

System Requirements

Conventional fluorescence microscopy Microscope:

Axioplan 2 imaging MOT,Axiovert 200 M, Axioskop 2 MOT

Digital Camera:AxioCam (monochrome)Computer:Min. Pentium III, 900 MHz,512 MB RAM

Software:

AxioVision Release 3.x with Z-stack module. Optional modules:Multichannel, Time-lapse,Mark&Find

Confocal microscopy System:

LSM 510 or LSM 5 PASCAL,software version 2.8 or later