SCL基因重组慢病毒上调高糖环境下Cajal间质细胞中ckit蛋白表达
中南大学学报（医学版）J Cent South Univ (Med Sci)2019, 44(2) htt p://https://www.wendangku.net/doc/9b11311731.html, 117S CL 基因重组慢病毒上调高糖环境下
Cajal 间质细胞中c-kit 蛋白表达
申茂磊１，钱彪１，徐浩２，傅家豪１，王昱琛１，王勤章１
（ １． 石河子大学医学院第一附属医院泌尿外科，新疆 石河子 ８３２０００；２． 解放军６９２３５部队卫生所，新疆 奎屯 ８３３２００ ）［摘要］目的：探讨含人干细胞白血病(stem cell leukemia ，SCL)基因重组慢病毒对高糖环境下受损Cajal 间质细胞(interstitial cells of Cajal ，ICC)中酪氨酸激酶受体(c-kit)蛋白表达的影响。方法：分离ICC ，培养24 h 贴壁，经倒置显微镜、免疫荧光鉴定后，将ICC 细胞分为对照组、高糖组，对照组加入含5 mmol/L 葡萄糖的培养基，高糖组加入含20 mmol/L 葡萄糖的培养基，培养48 h 后；对照组不变，高糖组分成空白组、空慢病毒组和实验组3个亚组，3组分别加入PBS 、空慢病毒和含SCL 基因重组慢病毒5 mmol/L 葡萄糖的培养基，继续培养24和48 h ，采用Western 印迹法检测两个时段各组中ICC 中c-kit 蛋白表达情况。结果：24或48 h 后，空白组、空慢病毒组中ICC 的c-kit 蛋白表达水平均明显低于对照组，实验组中ICC 中c-kit 蛋白的表达水平明显均高于空白组和空慢病毒组，但是仍低于对照组(均P <0.05)。结论：SCL 基因重组慢病毒可以上调高糖环境下功能受损ICC 中c-kit 蛋白表达量。
［关键词］ 干细胞白血病基因重组慢病毒；高糖；Cajal 间质细胞；c-kit 蛋白
E ffect of recombinant lentivirus of SCL gene on expression
of c-kit protein in interstitial cells of Cajal
under high glucose condition
S HEN Maolei 1, QIAN Biao 1, XU Hao 2, FU Jiahao 1, WANG Yuchen 1, WANG Qinzhang 1
( 1. Department of Urology, First A? liated Hospital, Medical College, Shihezi University, Shihezi Xinjiang 832000;
2. Health Center, PLA 69235, Kuitun Xinjiang 833200, China )
ABSTRACT O bjective: To determine the effect of a recombinant lentivirus containing human stem cell
leukemia (SCL) gene on the expression of c-kit protein in damaged interstitial cells of Cajal (ICC)
under high glucose condition.
Methods: Aft er isolation of ICC, the cells were cultured for 24 hours until the cells were adherent.
Aft er identifi cation by inverted microscope and immunofl uorescence, ICC cells were divided into
收稿日期(Date of reception)：2018-04-28
第一作者(First author)：申茂磊，Email: 4372943711@https://www.wendangku.net/doc/9b11311731.html,, ORCID: 0000-0001-6275-4359
通信作者(Corresponding author)：王勤章，Email: wqz1969@https://www.wendangku.net/doc/9b11311731.html,, ORCID: 0000-0002-7202-3282
基金项目(Foundation item)：国家自然科学基金(81360120)。Th is work was supported by the National Natural Science Foundation of China (81360120).DOI:10.11817/j.issn.1672-7347.2019.02.001
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