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JBC要求

JBC要求
JBC要求

Reports must be no longer than 30,000 characters including spaces, 2 figures and/or tables, but excluding references. For every figure or table over two, an additional 2,000 characters must be removed from the text. References should include titles

Overview of submission and review process The following is a brief overview of the submission and review process for JBC papers, along with links to the relevant instructions.

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Before preparing the manuscript

Research suitable for submission

Please consult the editorial policies before preparing your manuscript.

Manuscripts may be declined without a full review if they are clearly inconsistent with these guidelines.

Submitted manuscripts must describe original research not previously published and not under consideration for publication elsewhere. If related research is in press or under editorial review elsewhere, the abstracts or electronic reprints of these manuscripts should accompany your submission to JBC.

Manuscripts must be written in English, although papers may be submitted by authors from any country. Authors who are not native English speakers may appreciate

assistance with grammar, vocabulary, and style when submitting papers to the JBC. Several companies provide revising, editing, and proofreading services for scientific and medical research documents, including:

Please note that neither the JBC nor ASBMB has used these services and cannot attest to the quality of their work.

Copyright and co-author consent

All listed authors must concur in the submission and the final version must be seen and approved by all authors. All authors must transfer copyright to the American Society for Biochemistry and Molecular Biology.

JBC article types

JBC publishes several types of articles but only two of those can be submitted as an unsolicited manuscript: regular papers and reports. Most articles should be submitted as regular papers.

Reports (formerly known as Accelerated Publications) are concise but complete papers that present information on topics of exceptional novelty, significance, and broad interest to readers of the JBC. To undergo expedited review and be featured as a report, the quality of an article must fall within the top 5 percent of all articles published in the journal. Reports are not simply short versions of regular papers, and the existence of competing manuscripts under concurrent review is not sufficient to merit report status. Articles that do not meet the criteria for a report may be returned to authors without a full review. Reports must be no longer than 30,000 characters including spaces, 2 figures and/or tables, but excluding references. For every figure or table over two, an additional 2,000 characters must be removed from the text. References should include titles.

Publication charges

JBC announced a new pricing structure, effective January 1, 2013.

ASBMB member publication charges*

Pages: $80 per journal page

Color figures: FREE

*Member discount applies if the corresponding author is an ASBMB regular member. Standard (non-member) publication charges

Pages: $90 per journal page

Color figures: $50 per figure

Note on color figures: We encourage authors to use color figures where they will enhance the presentation of the data. Any figure submitted in color will be reviewed and processed with the understanding that the figure will be published in color.

Reprints: Pricing is based upon the quantity ordered.

All articles, including NIH-funded ones, are automatically deposited in PubMed Central. More information on JBC and PubMed.

The authors must pay all page and color fees. ASBMB will consider requests for waiver of publication charges if research funds are not available for publication costs. Waiver requests must be made at the time of submission and the request must be co-signed by an institutional official certifying that the authors do not have research funds available for publication costs.

Author Choice option: In addition to the standard publication charges, for the following additional publication fee — $1,500 for ASBMB members and $2,000 for nonmembers — the final version of your manuscript will be released immediately on the publisher’s website and PubMed Central and will be covered under the Creative Commons Attribution license (CC BY). Click here for details.

Note: If the Author Choice option is selected after publication, an additional $250 fee applies.

Join ASBMB NOW!

Click here to return to the top of the page. Preparing manuscript text

Text formatting

Prepare the text in Microsoft Word 6.0 (Word 2001 for Mac) or a later version. Format the manuscript similar to a published JBC paper because, if your manuscript is accepted, a PDF version of it will be published directly upon acceptance as a Paper in Press. The manuscript should have the approximate appearance and layout shown in the example paper below. Click on the paper below to view the complete example paper.

Form atting requirements:

?8.5-by-11-inch paper size (standard US letter).

?Single-spaced text throughout.

?Two-column format for capsule/abstract through discussion sections. Single-column format for title, references, footnotes, figure legends and tables. Click the image above to see an example.

See below for help converting text to columns in Microsoft Word.

?One-inch left and right margins and 0.25-inch spacing between columns.

?11-point Times New Roman font.

?Number all pages, including those with figures. Manuscripts without page numbers will be returned to authors for correction before review, thereby delaying the review process. Converting text to columns in Microsoft Word:

?Finish writing and editing the text.

?Select text in the capsule/abstract through discussion sections.

?Click on ―Format,‖ and then ―Columns‖ from the drop-down menu.

?Select two columns and equal column width, and then change the spacing to 0.25 inches.

Click here to return to the top of the page. Text Organization

Order of Sections

Manuscripts should be arranged in the following order:

a.title, author(s), complete name(s) of institution(s) and running title

b.capsule

c.abstract

d.introduction

e.experimental procedures

f.results

g.discussion

h.references

i.footnotes

j.figure legends

k.tables

l.figures

Title

?Should be intelligible to JBC readers who are not specialists in the field and should convey your essential points clearly.

?Should be short and informative (less than two lines).

?Should avoid acronyms or abbreviations aside from the most common biochemical abbreviations (e.g., ATP). A complete list of abbreviations that are acceptable in titles can be

found on the abbreviations page. Other acronyms or abbreviations should either:

o be introduced in their full form (e.g., Visualization of Polarized Membrane Type 1 Matrix Metalloproteinase (MT1-MMP) Activity in Live Cells by Fluorescence Resonance

Energy Transfer (FRET) Imaging); or

o be clarified by use as a modifier of the appropriate noun (e.g., FOX1 transcription factor, ACC dopamine receptor).

Running title

?Should be at the top of each page and may not exceed 60 characters and spaces.

Authors

?All authors are responsible for the content of the manuscript.

?Provide the complete names of all authors.

?Identify which author will receive correspondence regarding the manuscript.

?Provide the corresponding author’s name, telephone and fax numbers, and current e-mail address.

?Non-Latin characters can be used for author’s names, as long as the characters can be encoded in Unicode (e.g., Chinese, Japanese, Korean, Arabic). These characters can only be used for author names, not author affiliations or titles. Non-Latin characters should be enclosed in parentheses after the transliterated version (see example below).

Key words

You must choose at least five keywords from this list. You may also choose up to five keywords that are not on the list. You may not choose more than ten keywords in total. Capsule

A capsule reports the core findings of a paper to a broad spectrum of readers in a way that makes clear how those findings significantly advance understanding of a biological process. The capsule and the abstract have different purposes and audiences, so the capsule is not simply a truncated version of the abstract. A

well-crafted capsule increases the impact of a JBC article by:

?Providing scientists outside your specialty the chance to be inspired by your work or to start an interdisciplinary collaboration.

?Communicating the value of your research to the public and policymakers.

?Helping teachers identify papers suitable for classroom instruction.

?Offering a succinct summary of your work that can be easily accessed from mobile devices.

How to write a Capsule

The capsule is a highly visible part of a JBC article so you should write it with care and

precision. The capsule:

?Must not exceed 60 words.

?Should use language with which all readers are familiar, including the broad scientific community and the general public.

?Use only approved acronyms. All others must be spelled out on first reference.

?Must have the following four sections:

Background: A complete sentence that explains the impetus and context of the work.

Results: A complete sentence that summarizes the major findings.

Conclusion: A complete sentence that summarizes the interpretation of the findings.

Significance:A complete sentence about the paper’s impact on the field and its

long-term implications.

?Each capsule section should add information, not repeat what has already been said.

?After you write the capsule, you should evaluate and revise it using these strategies:

o Honestly ask yourself whether non-specialists will understand your findings and their significance.

o Ask a non-specialist to read your capsule and then ask if they understood the

essence of your work.

Capsule Example 1

Background: A myloid fibrils are protein aggregates associated with numerous

neurodegenerative diseases.

Results: A theoretically consistent, two-parameter model is proposed describing very

distinct amyloid fibrillization kinetics.

Conclusion: A myloid fibril formation takes place by a general mechanis m involving

supersaturation-dependent nucleation and growth steps.

Significance: This mathematically simple model can be routinely used to

characterize the action of new targets for disease therapeutics.

Capsule Example 2

Background: Galectins from peritoneal cells of conger eel contribute to the

encapsulation of nematode.

Results: A new galectin from peritoneal cells, congerin P (Con-P), shows unusual

sequence, specificity, and allosteric regulation by mannoside.

Conclusion: Con-P is a new type of galectin with allosteric carbohydrate-binding

ability.

Significance: Con-P is the first known lectin allosterically modulated by its ligands.

Capsule Example 3

Background: Metabolite binding to riboswitch RNAs regulates expression of

metabolic genes.

Results: Inhibitory and activating ligands interact with the same riboswitch.

Conclusion: Riboswitches integrate information about the overall metabolic state of

the cell.

Significance: This might be the first sign of a complex RNA-metabolite interactome.

Click here to return to the top of the page.

Abstract

?Should succinctly and clearly describe the major findings reported in the manuscript.

?Avoid specialized terms and use only approved acronyms. All others must be spelled out on the first reference.

?Must not exceed 250 words.

Introduction

?Presents the purpose of the study and its relationship to earlier work in the field.

?Should not be an extensive review of the literature.

?Usually less than one formatted page.

Experimental procedures

?Brief but sufficiently complete to permit a qualified reader to repeat the experiments.

?Only truly new procedures should be described in detail.

?Previously published procedures should be referenced.

?Modifications of previously published procedures should not be given in detail except where necessary to repeat the work.

?If the study characterizes the activity of new compounds, compound structures must be provided.

?Quantification of gel or blot intensities must be performed with data obtained within a linear range of exposure.

Results

?Presented in figures, tables, or text.

Discussion

?Concise (usually less than two formatted pages).

?Focused on the interpretation of the results

?Should not repeat information in the ―Results‖ section.

References

?Cited in text by number only.

?References should include article titles.

?Numbered consecutively in the order of appearance.

?If you use EndNote version 5 or earlier, you must update the JBC EndNote style. (See Updating EndNote, below, for instructions.)

?When using the EndNote plugin in Microsoft Word, you will have to manually add the DOI after the journal title for all e-pub references (see reference example 3).

?Journal names are abbreviated according to PubMed. (After updating your JBC style in EndNote, in order to ensure proper journal name abbreviations, see Updating EndNote, below, for

instructions.)

?Authors are responsible for the accuracy of the references.

Examples:

1.MacDonald, G. M., Steenhuis, J. J., and Barry, B. A. (1995) A difference Fourier

transform infrared spectroscopic study of chlorophyll oxidation in

hydroxylamine-treated photosystem II. J. Biol. Chem.270, 8420–8428

2.Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory

Manual, 2nd Ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

3.References appearing as e-pubs should be in the following style: Aphasizheva, I.,

Aphasizheva, R., and Simpson, L. (April 1, 2004) RNA editing terminal uridylyl

transferase 1: identification of functional domains by mutational analysis. J. Biol.

Chem.10.1074/jbc.M401234200

4.Farrell, C. (1992) The Role of SecB during Protein Export in Escherichia coli. Ph.D.

thesis, The Johns Hopkins University

Updating EndNote:

EndNote versions 5 and earlier do not contain the current JBC reference style with titles. To

obtain the current EndNote style:

?Visit https://www.wendangku.net/doc/aa7270983.html,/downloads/style/journal-biological-chemistry.

?Download the file J Biological Chem.ens. Save it in your EndNote Style folder so that it replaces the current JBC style.

?Full instructions for installing the new ens file can be found on the right-hand side of the Web page https://www.wendangku.net/doc/aa7270983.html,/downloads/styles.

?For the journal name abbreviations to appear correctly as EndNote renders them in your manuscript text document (e.g., ―J.Biol.Chem.‖), you will need to ensure that the

correct term list is imported. Follow the steps listed at https://www.wendangku.net/doc/aa7270983.html,/kb/82228.

Click here to return to the top of the page.

Unpublished observ ations and personal communications

?Must be cited using footnotes.

?Authors must obtain written approval for all personal communications, exactly as written in the text, to be provided to the Editor upon request.

Abbreviations

?Must be defined in a single footnote inserted after the abbreviation is first used.

?Some common biochemical abbreviations —e.g., ATP, NADH, DNA, and amino acids in proteins — need not be defined. A complete list of abbreviations that are acceptable in the text is available on the abbreviations page.

?Names of enzymes are usually not abbreviated except in terms of the substrates for which there are accepted abbreviations —e.g., ATPase and RNase.

?The trivial and systematic names of enzymes should be those recommended by the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology (IUBMB).

?Table I lists the abbreviations for units of measurement and certain physical and chemical quantities that can be used without definition. Also listed are the prefixes that can be added to names of units and the multipliers indicated by each prefix.

Table I: Abbreviations of units of measurement and of physical and chemical quanities.

Genetic nomenclature

Organisms should be described using standard nomenclature following the guidelines developed by the American Society for Microbiology

(https://www.wendangku.net/doc/aa7270983.html,/site/misc/journal-ita_nom.xhtml#04).

Chemical and mathematical usage

?Numerical data should be reported with the number of significant digits that corresponds to the magnitude of experimental uncertainty.

?Chemical equations, structural formulas and mathematical equations should be placed between successive lines of text.

?For lipids, please refer to the classification, nomenclature, and structural representation of lipids used by the LIPID MAPS Initiative (see Fahy et al. J. Lipid Res. 2005 46: 839–862). You can

download lipid structures directly from the LIPID MAPS Structure Database. This database

draws structures de novo, allowing you to insert them into your documents.

?In general, the rules and recommendations of the IUBMB and the International Union of Pure and Applied Chemistry (IUPAC) will be used for abbreviation of chemical names, nomenclature of chemical compounds, enzyme nomenclature, isotopic compounds, optically active isomers,

and spectroscopic data. Table II lists references to publications of the rules and

recommendations of the International Scientific Unions that may be consulted for detailed

information.

Table II: Tentative rules and recommendations of

international scientific unions.

Many of the documents in this table can be obtained

at https://www.wendangku.net/doc/aa7270983.html,/iupac/index.html.

Chemicals

Papers describing the activity of synthetic or natural chemical entities must include the chemical structures of such molecules, either as systematic names or as drawn structures. For synthetic chemical entities, a protocol for synthesis should be provided, generally as an allowable data supplement. Alternatively, reference to a publication or

issued patent that includes such a synthetic scheme and details can be provided. For natural chemical entities, methods for extraction/purification of the chemical entity, plus determination of the chemical structure, should be provided in the Materials and Methods section.

Enzyme activity data

Papers reporting kinetic and thermodynamic data concerning enzymes and other catalytic proteins and nucleic acids should include the identity of the enzymes, additional biological information (e.g., species and tissue normally found in,

post-translational modification), preparation and criteria of purity, assay conditions, methodology, activity, and any other information relevant to judging the reproducibility of the results. See the Beilstein Institut/STRENDA (standards for reporting enzymology data) commission Web site

(http://www.beilstein-institut.de/en/projekte/strenda/guidelines/) for more details and suggestions.

Enzyme activity (steady-state) generally should be reported in terms of V max (nmol or μmol product formed per amount ((enzyme)) per time) or, when possible, as

k cat (V max divided by molar enzyme concentration), in min?1 or s?1. Km units are given in molarity.

Any other units of activity (absorbance, % change) should be converted to units of molarity to express k cat or V max. Values of k cat (V max) and K m should be estimated using nonlinear fitting (and the software system cited).

Parameters should include estimates of error (e.g., SE). The use of linear transformation for Michaelis-Menten parameters is recognized to be inaccurate and use of an alternate method should be justified (e.g., graphical presentation of inhibition).

A lack of activity should be defined in terms of a limit of detection. In a series of comparisons to a basal or “control” level of activity (e.g., set as unity or “100%”), this activity should be indicated, in the units mentioned above, along with estimates of error. The inclusion of examples of some of the raw data is encouraged. Please refer to the STRENDA Web site regarding enzyme inhibition

(http://www.beilstein-institut.de/en/projekte/strenda/guidelines/). K i values are preferred to IC50.

Protein and nucleic acid sequences

Newly determined nucleotide or protein sequences must be deposited in GenBank (https://www.wendangku.net/doc/aa7270983.html,/genbank/), EMBL (https://www.wendangku.net/doc/aa7270983.html,), or the DNA Databank of Japan (http://www.ddbj.nig.ac.jp). Accession numbers must be reported in the manuscript and data must be available upon acceptance and publication of the manuscript as a Paper in Press. No data are to be withdrawn following publication.

Genomic and proteomic data

Authors of papers that include functional genomics data such as microarray, ChIP seq, RNA-seq, or other high-throughput data are required to deposit the data in a MIAME-compliant database such as GEO (https://www.wendangku.net/doc/aa7270983.html,/geo), ArrayExpress (https://www.wendangku.net/doc/aa7270983.html,/arrayexpress) or CYBEX (http://cibex.nig.ac.jp/data/index.html) and to provide accession numbers. Data must be publicly accessible upon acceptance and publication of the manuscript as a Paper in Press. No data are to be withdrawn following publication.

Authors of papers that include proteomics data should comply with the guidelines developed by Molecular and Cellular Proteomics

(https://www.wendangku.net/doc/aa7270983.html,/site/misc/CheckList.pdf).

Database accession hyperlinks

The journal provides direct hyperlink access to entries in databases like GenBank. Authors must provide database accession numbers for all new genomics and proteomics data reported in their manuscripts. In addition, authors are strongly encouraged to include accession numbers for any database information that would aid a reader in understanding the paper, regardless of who deposited the information. For database hyperlinks to be generated automatically, the citation must appear as a footnote and be written as follows:

1.GenBank = GenBank Accession Number XYYYYY

2.Molecular Modeling Database = MMDB # XYYY

3.NCBI Protein Database = NCB Accession # XXXXX

4.UniProtKB = UniProt # XXXXXX

5.Enzyme Collection Number = xx.yy.zz.bb

6.Research Collaboratory for Structural Bioinformatics Protein Databank = PDB # XXXX

The molecule or structure for an accession number can be identified through the NCBI Entrez utility at https://www.wendangku.net/doc/aa7270983.html,/sites/gquery.

Example:

The nucleotide sequence for the artificial sperm whale myoglobin gene has been deposited in the GenBank database under GenBankAccession Number (Reference). The amino acid sequence of this protein can be accessed through NCBI Protein Database under NCBI Accession # 2311060 (Reference). The atomic coordinates for the crystal structure of this protein are available in the Molecular Modeling Database

(https://www.wendangku.net/doc/aa7270983.html,/sites/entrez?db=structure) under MMDB # 5MBN(Reference). Authors may not include homepage URLs or use URLs to refer readers to unpublished

data.

Structural studies

For papers describing structures of biological macromolecules, the atomic coordinates and the related experimental data (structure factor amplitudes/intensities and/or NMR restraints and chemical shifts) must be deposited at a member site of the Worldwide Protein Data Bank, RCSB PDB, PDBe, PDBj, or BMRB.

The PDB ID assigned after deposition should be included in the manuscript. Authors must also submit the PDB Summary Validation Report (provided after annotation by the wwPDB) to JBC for review at the time of submission.

PDB data must be ready for release before final acceptance of the manuscript. No data are to be withdrawn from PDB once a paper has been acc epted and published as a Paper in Press article. As of December 2006, PDB no longer accepts coordinates for model structures determined by computational methods. The coordinates must be included as a supplement to the online paper and formatted just as if it were a PDB submission.

Click for additional guidelines for structural studies.

Click here to return to the top of the page. Preparing tables & figures

Introduction

The number of tables and figures used to present data essential to illustrate or prove a point should be kept to a minimum. Read the following instructions and the guidelines at https://www.wendangku.net/doc/aa7270983.html,/da/jbc/guidelines.html carefully to avoid publication delays. If you require further information, please contact jbc@https://www.wendangku.net/doc/aa7270983.html,.

Tables

Tables should have titles and sufficient experimental detail in a legend immediately following the title to be understood without reference to the text. Each column in a table must have a heading. Abbreviations, when necessary, should be defined in the legend. Complex tables, such as sequence alignments, should be submitted as figures. Very large tables that cannot fit on a single page should be submitted as supplemental information in an appropriate format, such as Excel.

Figures

Policy on image m anipulation

While certain modifications of primary data are often needed for clarity and/or brevity, image manipulation for deceptive purposes, to unfairly enhance or eliminate or otherwise obscure data, is misconduct and will be addressed as such.

For graphic material, we have adopted the policy of The Journal of Cell Biology:

“No specific feature within an image may be enhanced, obscured, moved, removed, or introduced. The groupings of images from different parts of the same gel, or from different gels, fields or exposures must be made explicit by the arrangement of the figure (e.g., using dividing lines) and in the text of the figure legend. Adjustments of brightness, contrast, or color balance are acceptable if they are applied to every pixel in the image and as long as they do not obscure, eliminate, or misrepresent any information present in the original, including the background. Nonlinear adjustments (e.g., changes to gamma settings) must be disclosed in the figure legend.”

File formats

You will initially submit the figures in a single PDF file with the manuscript text for review. If your paper is accepted, you will need to submit the figures as high resolution TIFF or EPS files. For tips on how to prepare high resolution TIFF or EPS files,

visit https://www.wendangku.net/doc/aa7270983.html,/da/jbc/index.jsp.

It is your responsibility to verify the quality of the graphics and confirm that compression of the files during the submission process does not distort the images. We cannot accept figure files in certain applications, such as Microsoft Office (PowerPoint, Word, Excel, Access), Corel Perfect Office (WordPerfect, Quattro Pro, Presentations), and Lotus SmartSuite (Freelance Graphics, 1-2-3, Approach, and WordPro). These applications are not intended for high-resolution imaging.

Color figures

We encourage the use of color figures where they will enhance the presentation of the data. Note that each color figure carries a $50 publication charge if the corresponding author is not a member of ASBMB, but will be published at no additional charge if the corresponding author is an ASBMB member. Any figure submitted in color will be reviewed and processed with the understanding that the figure will be published in color.

Color figures must be prepared in RGB color mode, not CMYK. JBC uses the RGB format to optimize color performance for online publication.

Multipanel figures

The parts of multipart figures (i.e., figures with parts labeled a, b, c, d, etc.) should be assembled into a single figure that fits on one page and should be provided as a single file. That is, rather than providing separate files for each panel (e.g., Fig1a, Fig1b, Fig1c,

Fig1d, etc.), please send just one file for each composite figure.

Figure dimensions

Figures should be properly sized and cropped so that no unnecessary white space is

left bordering the figure. Figures are sized to fit 1, 1.5 or 2 column widths. See Table III

for column dimensions. The maximum height should be 6 inches (15 cm).

Table III: Maximum figure widths

Units1-column width 1.5-column width2-column width

cm8.9 12.7 18.2

inch 3.5 5 7.2

pica21 30 43 Titles and legends

Figures should have titles and legends containing sufficient detail to make the figure easily understood. Legends should be organized consecutively in a separate section of

the manuscript. Indicate the figure number on each figure.

Letters, numbers and symbols

It is important to choose letters with the appropriate proportion and size. Figures in

the Journal are sized in 1-column, 1.5-column, or full 2-column widths (see Table III above for dimensions). Your figures will be sized so that the smallest type size on the figure will be at least 2mm.

Numbers, letters and symbols used in multi-paneled figures must be consistent. In the examples below, the poor quality figure has a wide range of text sizes and the good quality figure has all the text in proportion.

Poor quality figure

(inconsistent text size and unlabeled graph

axes)

Click on the image to see a larger version

Good quality figure (consistent text and labeled graph axes) Click on the image to see a larger version

The following fonts are recommended for use in figures:

European PI

Helvetica

Mathematical PI

Times Roman

Symbol

Arial

Graphs

The axes should be clearly labeled with appropriately sized type, and units of measurement must be given. Failure to comply with these specifications will require new figures and delay publication. Use standard symbols found in Microsoft Word. Define all symbols and curves in the figure or the legend. Equations used to plot lines and curves should be given in the legend or the text. Scales for plotting the data should be marked by short index lines, but every index line need not be numbered.

Image resolution

It is extremely important that figures be prepared with the proper resolution for publication in order to avoid inaccurate presentation of the data. The minimum

acceptable resolution for all figures is 300 dpi. Excessive file compression can distort images, so files should be carefully checked after compression. Note that figures that contain both line art (such as graphs) and RGB/grayscale areas (such as photographs) are best prepared as EPS (vector) files with embedded TIFF images for the

RGB/grayscale portions. The resolution of those embedded TIFF images should be at least 300 dpi.

Additional requirement for EPS files

If you supply your figures in EPS format you should convert all fonts to outlines/paths to avoid problems with character substitutions. To convert fonts to outlines in Adobe Illustrator, choose Edit > Select All then Type >Create Outlines.

Testing figure quality with Rapid Inspector

You can use an application called Rapid Inspector to check your TIFF or EPS files against JBC standards for format, resolution, color mode and other figure requirements. Download Rapid Inspector here.

Click here to return to the top of the page.

Allowable supplemental data

Now that the JBC is published exclusively online, division of a paper’s contents between a core article and an “online supplement” is largely anachronistic. Manuscripts should be complete and self-contained. Supplemental information must be limited to such things as videos, 3-D structures/images, extended chemical syntheses, extensive NMR data, molecular dynamics, kinetic modeling data, and other large data sets —e.g., those obtained with microarray analyses or mass spectrometry studies. All regular figures and tables (e.g., if they would fit on one redacted page) should be included in the main body of the manuscript and not in a supplemental file. Manuscripts that contain inappropriate supplemental information will be returned to authors for correction, thereby delaying the review process. Supplemental data will be reviewed as a part of the normal manuscript review process and will be judged by the same rigorous criteria as the main body of the paper. Allowable supplemental data should be submitted as a single file, if possible, that begins with the title of the article and the names of the authors and includes the legends, if any. Supplemental information cannot be added after acceptance.

Movies and large Excel files should be submitted in their native formats. Links to author or third-party sites are not allowed because they lack perma nence, though links to official databases like the Protein Data Bank (PDB) are encouraged (see Protein and nucleic acid sequences and Genomic and proteomic data sections above). The following supplemental data file formats are accepted:

avi mov txt

doc mpg wmv

docx pdb xls

gif pdf xls x

html ppt zip

jpg pptx

3-D images embedded in PDFs can be included in manuscripts. Please alert the JBC

office by e-mail at the time of submission if you plan to submit a 3-D image so that

the files can be handled properly (this option will be offered to you during the

submission process). If the file size is too large for the system, JBC staff can make

special arrangements. See here for detailed information on how to create 3-D files for submission.

Preparing cover letter

Please provide a cover letter that includes a brief description of the novelty and significance of the research (two to three sentences.) Please note whether any version

of this manuscript has been submitted to the JBC previously and provide any previous manuscript number(s). Let us know about any previous communications with a JBC

editor or staff member regarding this manuscript. You are encouraged to suggest appropriate reviewing editors with the expertise needed to evaluate your manuscript.

You may also suggest an appropriate associate editor to handle the review of your manuscript, although the Editor will make the final decision. Information about our associate editors and reviewing editors can be found through this

link: https://www.wendangku.net/doc/aa7270983.html,/jbcboard.

Preparing manuscript PDF for submission

Once you have finished preparing the manuscript, convert the text and figure files (but

not supplemental data) into a single PDF with a file size of less than 10 MB. You can

create the PDF using Adobe Acrobat, which is available for

purchase https://www.wendangku.net/doc/aa7270983.html,/products/main.html. Microsoft Word also has a "Save As" feature

that will allow you to save a file in PDF format. (PC versions 2007 and higher,

Macintosh 2004 and higher.) Be aware that figure resolution may be reduced if this

option is used. To review your image resolution settings, please refer to the help

section of Microsoft Word regarding "Compress Pictures."

Consult the Adobe Acrobat manual for detailed instructions on creati ng PDF files. We recommend the following settings:

?Text and Graphics LZW Compression ON

?Thumbnail Generation OFF

?Generating ASCII format Files OFF

?Subset Embedded Fonts ON

?Color Image Downsampling ON

?Color Image Downsampling Resolution 150

?Color Image Compression ON

?Color Image Compression JPEG Medium

?Grayscale Image Downsampling ON

?Grayscale Image Downsampling Resolution 150

?Grayscale Image Compression ON

?Grayscale Image Compression JPEG Medium

?Monochrome Image Downsampling ON

?Monochrome Image Downsampling Resolution 300

?Monochrome Image Compression ON

?Monochrome Image Compression CCITT Group 4

File requirements:

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检验体。 6检验inspection 对检验项目中的性能进行量测、检查、试验等,并将结果与标准规定要求进行比较,以确定每项性能是否合格所进行的活动。 7见证取样检测evidential testing 在监理单位或建设单位监督下,由施工单位有关人员现场取样,并送至具备相应资质的检测单位所进行的检测。 8交接检验handing over inspection 由施工的承接方与完成方经双方检查并对可否继续施工做出确认的活动。 9主控项目dominant item 建筑工程中的对安全、卫生、环境保护和公众利益起决定性作用的检验项目。10一般项目general item 除主控项目以外的检验项目。 11抽样检验sampling inspection 按照规定的抽样方案,随机地从进场的材料、构配件、设备或建筑工程检验项目中,按检验批抽取一定数量的样本所进行的检验。 12抽样方案sampling scheme 根据检验项目的特性所确定的抽样数量和方法。 13计数检验counting inspection 在抽样的样本中,记录每一个体有某种属性或计算每一个体中的缺陷数目的检查方法。 14计量检验quantitative inspection 在抽样检验的样本中,对每一个体测量其某个定量特性的检查方法。 15观感质量quality of appearance

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