Column:
Zenix ? SEC-300 (3 μm,300 ?, 7.8x300 mm) Mobile Phase: 150 mM phosphate buffer, pH 7.0 Flow Rate: 1.0 mL/min; Temperature: 25 o C
Detection:
UV 280 nm Injection Volume: 10 μL
Samples: Bevacizumab (trade name Avastin), a humanized monoclonal antibody, 150 kD
Analysis of Bevacizumab and Erbitux Monoclonal Antibodies on
Zenix ? SEC-300 (7.8 x 300 mm)
A U
0.00
0.05
0.10
0.15
0.20
0.25
Minutes 1.00
2.00
3.00
4.00
5.00
6.00
7.00
8.00
9.0010.0011.0012.0013.0014.00
Column: Zenix? SEC-300 (3 μm,300 ?, 7.8x300 mm)
Mobile Phase: 20 mM sodium phosphate buffer(pH7.0), 0.3 M NaCl Flow Rate: 1.0 mL/min;
Temperature: 25 °C
Detection: UV 214 nm
Injection Volume: 10 μL
Samples: Erbitux (humanized monoclonal antibody) Keywords: Size exclusion, Zenix, monoclonal antibody, bevacizumab, erbitux