贝伐单抗和艾比特思单抗在体积排阻色谱柱上的分离图谱

Column:

Zenix ? SEC-300 (3 μm,300 ?, 7.8x300 mm) Mobile Phase: 150 mM phosphate buffer, pH 7.0 Flow Rate: 1.0 mL/min; Temperature: 25 o C

Detection:

UV 280 nm Injection Volume: 10 μL

Samples: Bevacizumab (trade name Avastin), a humanized monoclonal antibody, 150 kD

Analysis of Bevacizumab and Erbitux Monoclonal Antibodies on

Zenix ? SEC-300 (7.8 x 300 mm)

A U

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0.25

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9.0010.0011.0012.0013.0014.00

Column: Zenix? SEC-300 (3 μm,300 ?, 7.8x300 mm)

Mobile Phase: 20 mM sodium phosphate buffer(pH7.0), 0.3 M NaCl Flow Rate: 1.0 mL/min;

Temperature: 25 °C

Detection: UV 214 nm

Injection Volume: 10 μL

Samples: Erbitux (humanized monoclonal antibody) Keywords: Size exclusion, Zenix, monoclonal antibody, bevacizumab, erbitux