油菜和新疆野芥体细胞杂种后代的菌核病抗性鉴定

收稿日期:2007-07-15

基金项目:国家“863项目”(2006AA100106);湖北

省重大科技攻关项目(2006AA206A01);国家科技支撑项

目(6B D )

作者简介孙盼盼(8),女,辽宁省抚顺人,硕

士研究生研究方向作物遗传育种3通讯作者油菜和新疆野芥体细胞杂种后代的菌核病抗性鉴定

孙盼盼1,李云昌1,梅德圣1,周　梁2,李英德1,徐育松1,胡　琼13

(11中国农业科学院油料作物研究所/农业部油料作物遗传改良重点实验室/国家油料作物改良中心,湖北武汉　430062;21孝感学院生命科学技术学院,湖北孝感　432000)

摘　要:菌核病是我国油菜的主要病害,发病严重年份可以造成10%以上的产量损失.寻找菌核病抗源、选育抗病品种一直是油菜育种的重要课题.新疆野芥分布于我国西北部新疆地区,具有较好的抗真菌性病害能力.利用离体叶接种、牙签法侵染和大田自然鉴定等方法,对新疆野芥与甘蓝型油菜的体细胞杂种后代衍生株系进行了抗菌核病鉴定试验.结果表明,体细胞杂种后代的抗病性高于对照品种中油821、中双4号和中双9号.并通过测定PAL ,POD 和PPO 活性的变化来研究抗病材料的抗性机理.

关　键　词:油菜;体细胞杂交;菌核病;抗性品系

中图分类号:S634130314文献标识码:A

Deter mina tion o f stem r ot r esistance of lines der ived f r om

somatic hybr ids bet w een Br assica napus and Si napis arvensis

S UN Pan 2pa n 1,L I Yun 2chang 1,MEI De 2sheng 1,ZHOU L iang 2,

L I Ying 2de 1,XU Yu 2song 1,HU Q iong 13

(11K ey Laboratory of Agricult ural Minist ry for Oil Crops Improvement/National Center for Oil Crops Improvement/Instit ut e of Oil Crop Research ,Chinese Academy of Agricult ural Sciences ,Wuhan 430062,Chi na ;21College of Life Science ,X iaogan Universit y ,X iaogan ,Hubei 432000,China )

Abstra ct :Stem rot is a major disease causing more tha n 10%yield loss in China during the years when the disease is severe 1Identification of resista nt res ources and develo pment of lines with high resista nce have been t he main objective of oilseed rape breeding 1X injia ng wild rape ,a popu lation of Sinapis arvens is distributed in Northwest China ,possesses high resistance to f ungal diseases 1Using inoculation methods ,including in oculating detached leaves a nd stems with mycelia ,combined wit h field ide nti f ication ,t he resistance of lines derived f rom somatic h ybrids between Brass ica napus and Sinapis arve nsis was investigated 1The result s sh owed t hat some of the lines were more resistant tha n Zhon gy ou 821,Zh ongshuang 9and Zhongshuang 4to S 1sclerotiorum ,1The possible mec hanism of resistance to Sclerotinia sclerotiorum 1The dynamics of enzyme activity of p henylalanine ammonialyase (PAL ),peroxidase (POD )

and

polyphenoloxidase (PPO )in the materials were stud ied to under stand the mec hanisms of the resistance 1

K ey w or ds :oilseed rape ;somatic hybridization ;S 1sclerotior um ;resista nt line 油菜品质改良是近二十多年油菜品种改良的

主要目标,不断有优质油菜品种选育成功.但我国的大部分优质油菜品种没能完全克服优质与高产、优质与抗病之间的矛盾[1].油菜菌核病是影响油菜高产的主要限制因子之一,在我国的平均发病率为%～3%,严重年份可达8%以上,导致减产第33卷

2007年8月湖南农业大学学报(自然科学版)Jour nal of Huna n Agricultural Univer s ity (Natural Sciences )Vol 133Aug 120078200A 07A04:192-.:.

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