pet15bm

Novagen ?ORDERING 800-526-7319 ?

TECHNICAL SUPPORT

800-207-0144

The pET-15b vector (Cat. No. 69661-3) carries an N-terminal His?Tag ?sequence followed by a thrombin site and three cloning sites. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA poly-merase is shown below.

pET-15b sequence landmarks

T7 promoter

453-469T7 transcription start 452His?Tag coding sequence 362-380Multiple cloning sites (Nde I - Bam H I)319-335T7 terminator

213-259lacI coding sequence (866-1945)pBR322 origin

3882

bla coding sequence

4643-5500

pET-15b Vector

TB045 12/98

(1216)(1230)(1397)(1427)(1627)(1722)

Sap I BspLU11 I AlwN I Ahd I (4713)

Bsa I

pET-15b Restriction Sites TB045 12/98

Novagen ?FAX608-238-1388 ?E-MAIL novatech@https://www.wendangku.net/doc/c54469913.html,