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pET-19b

pET-19b
pET-19b

Novagen ?ORDERING 800-526-7319 ?

TECHNICAL SUPPORT

800-207-0144

(948)

(1225)(1239)(1406)(1434)(1436)(1636)(1731)(1884)

Sap I BspLU11 I Bsa I TB049 12/98

pET-19b Vector

pET-19b sequence landmarks

T7 promoter

472-488T7 transcription start 471His?Tag coding sequence 366-395Multiple cloning sites (Nde I -Bam H I)319-335T7 terminator

213-259lacI coding sequence 875-1954pBR322 origin

3891

bla coding sequence

4652-5509

The pET-19b vector (Cat. No. 69677-3) carries an N-terminal His?Tag ?sequence followed by an enterokinase site and three cloning sites. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA poly-merase is shown below.

Eam1105 I AlwN I

TB049 12/98

pET-19b Restriction Sites

Novagen ?FAX 608-238-1388 ?E -MAIL novatech@https://www.wendangku.net/doc/d31791275.html,

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