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Sap I BspLU11 I Bsa I TB049 12/98
pET-19b Vector
pET-19b sequence landmarks
T7 promoter
472-488T7 transcription start 471His?Tag coding sequence 366-395Multiple cloning sites (Nde I -Bam H I)319-335T7 terminator
213-259lacI coding sequence 875-1954pBR322 origin
3891
bla coding sequence
4652-5509
The pET-19b vector (Cat. No. 69677-3) carries an N-terminal His?Tag ?sequence followed by an enterokinase site and three cloning sites. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA poly-merase is shown below.
Eam1105 I AlwN I
TB049 12/98
pET-19b Restriction Sites
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