高速逆流色谱结合硅胶柱色谱法分离制备干姜中的6-姜酚、8-姜酚、6-姜烯酚

高速逆流色谱结合硅胶柱色谱法分离制备干姜中的6-姜酚、

8-姜酚、6-姜烯酚

徐美霞;王晓;刘建华;耿岩玲;王岱杰;刘峰

【期刊名称】《山东科学》

【年(卷),期】2013(026)002

【摘要】通过实验建立了高速逆流色谱(HSCCC)结合硅胶柱色谱法分离制备干姜中6-姜酚、8-姜酚和6-姜烯酚的方法.干姜样品采用乙酸乙酯超声提取,提取物经过硅胶柱色谱粗分,选取其中A3和A4两个馏分进行高速逆流色谱分离,所得单一成分采用高效液相色谱(HPLC)检测纯度,并采用电喷雾质谱(ESI-MS)、核磁共振氢谱(1H NMR)对所得化合物的结构进行鉴定.研究结果表明,该方法简便快捷,所得产物纯度高、制备量大、分离效率好.%We constructed the method of isolating 6-gingerol, 8-gingerol and 6-shogaol from dry rhizome of Zingiber officinale Rose, by silica gel column chromatography and high-speed counter-current chromatagraphy based on experiments. We extracted the samples of Zingiber officinale Rose, by ethyl acetate ultrasonic extraction and then coarsely treated them by silica gel column chromatography. We further selected fraction A3 and A4 to be separated by high-speed counter-current chromatography( HSCCC). The purity of the obtained single ingredient was detected by high-performance liquid chromatography(HPLC) and the structure of the obtained compound was identified by electrospray ionization(ESI-MS) and 1H-NMR. Results show that the method has