所有质粒载体汇总

酿酒酵母表达载体

pYES2,pYES2/NT,pYES2/CT,pYES3,pYES6, pYCplac22-GFP,

酵母载体pAUR123,pRS303TEF,pRS304, pRS305,pRS306,pY13TEF,pY14TEF

pY15TEF, pY16TEF,

酵母基因重组表达载体pUG6, pSH47,

酵母单杂载体pHISi,pLacZi,pHIS2, pGAD424,酵母双杂交系统：酿酒酵母Y187, 酿酒酵母AH109;质粒pGADT7,pGBKT7 ;对照质粒pGBKT7-53 , pGBKT7-lam , pGADT7-T , PCL1，酿酒酵母菌株INVSc1,YM4271, AH109,丫187,丫190,

毕赤酵母表达载体

pPIC9K,pPIC9K-His,pPIC3.5K,pPICZalphaA,B,C,pPICZA,B,C,pGAPZ a

A,pAO815,pPIC9k-His,pHIL-S1,pPink hc ,

配套毕赤酵母Pichiapink,

毕赤酵母宿主X33, KM71 , KM71H , GS115,

原核表达载体pQE30,31,32,40,60,61,62等原核表达载体，包括pET系列，pET-GST, pGEX 系列(含GST标签)，pMAL 系列pMAL-c2x,-c4x,-c4e,-c5x,-

p5x,pBAD,pBADHis,pBADmycHis 系列，pQE 系列,pTrc99a,pTrcHis系列，

pBV220,221,222,pTXB 系列，pLLP-ompA,pIN-III-ompA

(分泌型表达系列),pQBI63 (原核表达带荧光)pET3a, pET 3d, pET 11a, pET 12a, pET 14b, pET 15b, pET 16b, pET 17b, pET 19b, pET 20b, pET 21a,b,d, pET 22b, pET 23a, pET 23b, pET 24a,b, pET 25b, pET 26b, pET 27b, pET 28a,b, pET 29a, pET 30a, pET 31b, pET 32a, pET 35b, pET 38b, pET 39b, pET 40b, pET 41a,b pET 42a, pET

43.1a,b pET 44a, pET 49b pET302,303 pET His,pET Dsb,pET GST,pET Trx pQE2, pQE9 pQE30,31,32, pQE 40 pQE70 pQE80L pQETirs system pRSET-A pRSET-B pRSET-C pGEX4T-1,-2,-3,5x-1,6p-1,6p-2,2tk,3c pBV220,221,222 pTrcHisA,B,C pBAD24,34,43 pBAD HisA,B,C pPi nPoi nt-Xa1,Xa2,Xa3 pMALc2x, p2x pBV220 pGEM Ex1, pGEM7ZF (+) , pTrc99A, pTwin1, pEZZ18 pkk232-8,pkk 233-

3,pACYC184,pBR322,pUC119 pTYB1,pTYB2,pTYB4,pTYB11 pBlueScript SK

(+) ,pBlueScript SK (-) pLLP ompA, pINIIIompA, pMBP-P ,pMBP-C,大肠杆菌冷激质粒：pColdI pColdII pColdIII pColdTF原核共表达质粒：pACYCduet-1,pETduet-

1,pCDFduet-1, pRSFduet-1 Takara公司大肠杆菌分子伴侣：pG-KJE8 pGro7 pKJE7 pGTf2 pTf16 大肠杆菌宿主细胞：DH5a JM101 JM103

JM105 JM107 JM109 JM110 TopIO ToplOF BL21 ( DE3) HB101 ER2529 E2566 C2566 MG1655 XL-10gold XL blue M15 JF1125 K802 SG1117 BL21 (AI ) BL21 (DE3) plysS TG1 TB1 DH5a (pir) Tuner (DE3) Bl21 codonplusRIPL Novablue (DE3) Rosetta Rosetta(DE3) Rosetta (DE3) plys Rosetta-gami (DE3)

Rosetta-gamiB(DE3) , Rosetta-gamiB( DE3) plysS Orgami(DE3) OrgamiB (DE3) HMS174 (DE3)

植物表达/RNAi 载体农杆菌pBI121,pBI121-GFP,pBI101,pBI221,pSN1301,

pUN1301,pRTL2 , pRTL2-GFP , pRTL2-CFP, pRTL2-RFP , pRTL2-YFP,pCAMBIA 1300, 1301, 1302,1303,1304,1305, 1381 Z,1391 Z,2300, 2301,3300,3301,pCAMBIA super1300,pCAMBIA super1300-GFP,pPZP212,pPZP2121,pPZP212-

GFP,pGDG,RNA载体pART27,pHANNIBAL,pKANNIBAL, pFGC5941,pTCK303, pTRV1,pTRV2, T-DNA插入载体(随机突变体库)pSKI015,pSKI074,真菌ATMT载体pBIG2RHPH2-GUS-GFP,pBHt1

枯草芽抱杆菌表达载体pWB980,pHT43,pHP13,pHP43,

pBE2,pMUTIN4,pUB110,pE194,pMA5, pMK3,pMK4,pHT304,pHY300PLK,

pBest502,pDG1363,pSG1154,pAX01, pSAS144,pDL,pDG148-stu,pDG641,

pAL12,pUCX05-bgaB,pHT01 ,配套菌株BS 168,WB600,WB800,WB700,

WB800N,1012,FZB42,1A747,广宿主质粒pVLT33

RNAi 基因沉默干扰敲除载体pSilencer1.0, pSilencer 2.1-U6 hygro, pSilencer 3.1-H1 hygro, pSilencer 3.1-H1 neo pSilencer 4.1-CMV neo, pSilencer 4.1-CMV puro pMIR-REPORT Luciferase RNAi 载体(oligoengine) pSuper-puro RNAi 逆转录病毒载体(clontech) : RNAi-Ready pSIREN-Retro Q, RNAi-Ready pSIREN-RetroQ-ZsGreen ( Luciferase shRNA Ann ealed Oligo nucleotide) RNAi 慢病毒载体(addgene : pLKO.1

哺乳动物表达载体pcDNA3.1+/-,pcDNA4/HisMax B , pSecTag2 A, pVAX1 , pBudCE4.1,pTracer CMV2, pcDNA3.1 (-) /myc-His A , pcDNA6-Myc/His B , pCEP4, pIRES, pIRESneo, pIRES hyg3, pCMV-myc, pCMV-HA , pIRES-puro3, pIRES-neo3,pCAGGS哺乳动物双杂交系统pACT , pBIND , pACT-MyoD , pBIND-ld , pG5luc,pCMV-BD, pCMV-AD, pBD-p53, pFR-luc,Cytotrap Two-Hybrid

System:pSos, pSos MAFB, pMyr蜕皮激素诱导系统pIND, pVgRxR,LacSwith II 哺乳动物诱导表达系统：pOPRSVI , pOPI3CAT, pCMVLacl,GeneSwitch System:pSwitch 哺乳动物表面展示系统:pDisplay,四环素调控系统(Invitrogen): pcDNA4/TO/Myc-His

A ，pcDNA4/TO/Myc-His

B ，pcDNA4/TO/Myc-His

C ，pcDNA4/TO/Myc-

His/LacZ，pcDNA6/TR 四环素调控系统(Clontech) : pTet-On, pTet-Off，

pTRE2,pRevTRE, pRevTet-On, pRevTet-off信号通路报告载体:pGAS-TA-Luc，pSTAT3-TA-Luc, pISRE-TA-Luc, pTA-Luc ，pI K B-EGFP，pNFAT-TA-Luc，pCaspase3-senso, pAP1 (PMA) -Luc;pGL4.26[luc2P/mi

nP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFA T-RE/Hygro]，

pGL4.75;p53-Luc，pAP-1-Luc，pNF- K B-Luc，pSRE-Luc，pFA2-Elk1，pFC-MEKK，pFR-luc,Gateway系统(invitrogen) pcDNA6.2-GWEmGFP-miR negative, pLenti 6/TR,pcDNA 6.2-GW EmGFP-miR，

乳酸菌表达载体及各种乳酸菌乳酸杆菌菌株，

pNZ8148,pLEISS,pMG36e,pBBR1MCS-5,pBBR1MCS-6,pRV610,pLEM415,pHY3

00PLK,分泌型乳酸菌表达载体pVE5523，pPG611.1,pPG612.1等和乳酸杆菌菌株宿主菌NZ9000,MG1363,Lactobacillus casei 1.539,Lactobacillus casei,acidophilus NCFM,1.2,Lactobacillus sakei 23K,L.pla ntarum,L.rham no sus

GG,B.coagula ns,Bifidobacterium bifidum,Bifidobacterium infan tis,Lactococcus lactis M17,1663,Lactobacillus reuterii

广宿主表达载体链球菌表达敲除载体假单胞菌表达载体pVLT33,pBBR1MCS-2,3,4,5,6, pJRD215,pJN105,pME6032,Cos 载体pLAFR3,pMP2444 (GFP) ,

pHY300PLK,pRT102,pRL1063a,转座子载体pUT-mi niT n5,pMGS100,

pWHM10,pKC1139,pSET152, pOJ260,pPG611.1,pPG612.1

腺病毒载体/慢病毒，逆转录病毒表达载体及包装包膜质粒，腺病毒系统

(Stratagene : pAdEasy-1,pShuttle-CMV,pShuttle,pAdTrack, pAdTrack-CMV, pShuttle-IRES-hrGFP-1、pShuttle-IRES-hrGFP-2、pShuttle-CMV-lacZ, pShuttle-CMV-EGFP-C,pXC1, pBHGE3,配套大肠杆菌BJ5183,293,293T cell line 腺相关病毒系统(Stratagen? : pAAV-MCS , pAAV-RC , pHelper, pAAV-LacZ，pAAV-IRES-hrGFP , pCMV-MCS ,慢病毒载

体:pLVX-DsRed-Mo no mer-N1,pLVX-IRES-ZsGree n1,pLVX-AcGFP1-N1 丄en ti6/v

5-EDST-EGFP,pWPXL, FUGW,pLe ntilox 3.7,RNAi-Ready pSIREN-Retro Q, RNAi-Ready pSIREN-Retro Q-ZsGree n, pSUPER.Retro-GFP/Neo,pSUPER-Retro-Neo, pSUPER.Retro-puro,PLNCX PLNCX2 pMSCV-HYG pMSCV- neo pMSCV-puro pLEGFP-C1 pLOX-CW-CRE pLOX-GFP-IRES-TK pRetroX-IRES-DsRedExpress, pLVX-IRES-mCherry 质粒载体。病毒包装：二质粒包装系统一：psPAX2 pMD2.G二质粒系统

二：pCMV-dR8.2 dvpr pCMV-VSVG 三质粒包装系统具有更高的包装效率：

pMDLg/pRRE pRSV-Rev pMD2.G Invitrogen 包装系统：PLP1 PLP2 PLP/VSVG

基因重组载体敲除载体自杀质粒RED重组系统载体pKD3,pKD13,pKD4,pCP20,pKD46, pCVD442,pk18mobsacB,p2NIL, pMAD （革兰氏阳性菌基因敲除），

pBT2,RN4220,pKC1139,pOJ260, pJQ200SK,pSET152,pWHM10, pk18mobsacB, pGOAL17,pGOAL19,基因打靶载体PL253，PL451，PL452，PL453,配套菌株

EL250,EL350,pMLM290,292, KJBAC1,pST1374

荧光蛋白表达载体荧光定位质粒报告基因载体pEGFP,pEYFP,pECFP,pDsRED, pmCherry,pAcGFP,hrGFP,ZsGreen pDsRed1-C1, pDsRed1-N1,

pDsRED-Monomer-N1, pEYFP-C1, pEYFP-N1, pEGFP-1，pEGFP-C1, pEGFP-N1, pEGFP-N3, pcDNA-EGFP1,pECFP-N1,pECFP-C1, pAcGFP1-1 pDsRED2-ER, pDsRED2-Mito, pDsRED2-Nuc，pDsRED2-Peroxi , pEYFP-Golgi, pEYFP-Mem, pEYFP-ER , pEGFP-Actin, pECFP-ER, pIRES2-EGFP, pLEGFP-C1, pLEGFP-N1，pDsRED-Express-N1,plRES2-DsRed,plRES-AcGFP1,pmCherry-C1,N1 启动子增强子报告载体（Promega,带有Luc荧光报告基因）：pGL3-basic，pGL3-control，pGL3-enhance，pGL3-promoter，pRL-TK，pRL-CMV，pRL-SV40,信号通路报告载体（Clontech） : pGAS-TA-Luc，pSTAT3-TA-Luc, pISRE-TA-Luc, pTA-Luc ，pI K B-EGFP，pNFAT-TA-Luc，pCaspase3-sensqr pAP1 （PMA）-Luc 信号通路报告载体（Promega :

pGL4.26[luc2P/mi nP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFAT-RE

/Hygro], pGL4.75 信号通路报告载体(Stratagene : p53-Luc, pAP-1-Luc, pNF- K B-Luc, pSRE-Luc, pFA2-Elk1 , pFC-MEKK , pFR-luc

昆虫表达载体杆状病毒表达载体系统pFastBac1,pFastBac1-Gus,pFastBacHT A

pFastBacHT-CAT,pFastBacDual,pBlueBacHis2 A DH10Bac配套大肠杆菌，pMIB v5-His B ,pIZT/V5-His ,

Taq,Pfu高表达载体菌株KOD, T4连接酶高表达质粒载体，高产基因工程菌株、酶

基因KOD,T4 kinase 激酶，Tth 反转录酶MMLV , Klentaq PCR酶，Cre酶，RNA inhibitor 抑制酶，DL2000 质粒

BiFC双分子荧光表达载体FRET系统载体pBiFC-VC155 , pBiFC-VN173 , pBiFC-bFosVC155, pBiFC-bFos( deltaZIP)VC155 , pBiFC-bjun-VN173 , pCE-BiFC-

VN173 , pCE-BiFC-VC155 ,

真菌表达载体黑曲霉米曲霉黄曲霉表达质粒pBC-hygro,pAN7-1,pBARGPE1,真

菌ATMT农杆菌介导转化载体pBHt1,pBIG2RHPH2-GFP-GUS等,

酵母单杂交三杂交载体,酵母双杂交系统pGBKT7 , pGADT7 , pCL1 , pGBKT7-53 , pGADT7-T , pGBKT7-Lam ,配套酿酒酵母AH109酵母单杂交系统

(Clontech) :pHISi,pLacZi,pHis2,pHIS2.1 酵母三杂交系统:pBridge, YBZ1 菌

株Cytotrap Two-Hybrid System( Stratagene : pSos, pSos MAFB, pMyr

噬菌体展示系统载体1.噬菌体展示抗体scFv质粒:pCANTAB5E宿主菌：TG1辅助噬菌体：M13K07可溶表达菌：HB2151 2.噬菌体展示抗体Fab质粒：pcomb3宿主菌：XL1-Blue辅助噬菌体：VCSM13

pEVOL,

pBudCE4.1,pBudCE4.1-EGFP,Zeoc in ,pEGFP-C3,2,1,pmCherry-C1,-N1,pLOVE-mi R302/367,pDsRed2-N1,pAcGFP1.1,TALE toolbox

kit,plRES-hyg3,plRES-Neo3,plRES-puro3,pSIREN-RetroQ-ZsGree n,GST 14-3-

3,pDsRed-Mo nomer-N1,M50 super8x

TOPFIash,pRK793,pCMV-tag5B,pCMV-tag2B , pCMV-N-HA ,

pUAST,pw-25,PAC-5.1-HISA , pSLIK-Neo,pCMV-myc-cyto,pCMV-myc-nuc,疫苗表达载体

pVAX1,pAdEasy-1,pShuttle,pAdTrack,pAdTrack-CMV,-polyA,pShuttle-EGFP,BJ51 83,pXC1,pBHGE3,pSUPER-retro-GFP-Neo,pORF-lacZ pORF-TK, pCOX2-

Luc,pcDNA3.1 （-）-myc-His A,pcDNA3.1-p300,plNP, pOprE, pCR2.1

TOPO,pLP1,pLP2,pLP/VSVG,Stbl3,ple nti6/V5-DEST,pLVX-shRNA1,2,pLVX-IRE S,tdTomato,FUW-teto-hOCT4,hSOX2,hMYC,hKLF4,FUW-M2rtTA,pSecTag2

A,pEYFP-C1,pEGFP-N1,-C1,pcDNA3.1-V5-His

A,B,C,pcDNA3.1-EGFP,pLEGFP-C1, a -MHC Promoter,Marc-145猴肾细胞，Sf9, BmN昆虫家蚕细胞，酵母载体

pYES2,pYES2.0,INVSc1,pYES3,pYES6,pRS41H,pRS303,304,305

306,pRS424,425,426,414,415,416,pY13,14,15,16,YBZ1,L4（酵母菌，pESC-

His,pYIP5,pHIS2,pHIS2.1,pHISi,pLacZi,酵母重组载体pUG6,pSH47植物载体pCAMBIA1300-EGFP,

pTCK303,pTRV1,pTRV2,pFGC5941,pUN1301,pSN1301,C58C1，R1601,AGL1 农杆菌感受态，原核载体pQE3,pQE30,31,32,pQE60,61,62,M15,DH10Bac,Origami

（B）DE3,XL1-Blue,Rosetta gamiB（DE3）pLysS,TKB1,ER2566，DB3.1，

JM105,TOP10,DH5a,DH5alpha lambda pir,C43 （DE3）菌株感受态，pTrcHis

A,B,C,pTWIN1,pTYB1,pTYB11,pACYC184,pMAL-c5x,c2x,p5x,c4x,pBV220,221,2 22,pCold l,ll」ll,TF,pET22a,pET29a,pET28a,pET39b,pET41a,pET42a,p15A,pET-sumo,pBAD His A,B,C,pBAD24,34,pETDuet-1,pRSFDuet-1,pCDFDuet-1,pACYCDuet-1,原核荧光pQBI63,pMP2444,链球菌表达、敲除载体pKC1139、pOJ260 pWHM3，PSET152, pVA981，多宿主载体pGOAL19,pBBR1MCS-2,-5,-

6,pDSK519,pLA2917，pRK2013, pVLT33,pMAD,RN4220金葡菌株，真菌载体pBARGPE1,pAN7-1,pBHt1,pBIG2RHPH2-GFP-GUS,乳酸菌载体

MG1363,pNZ8148,NZ9000,pNZ9530,pMG36e,枯草芽抱杆菌载体

pHT43,pWB980,pHT300PLK,pMA5,pUTminiTn5,巨大芽抱杆菌表达载体

pHIS1525,WH320菌谷氨酸棒状杆菌表达载体pEC-XK99E,敲除载体pk18mobsacB, pET载体

3a, 3d

3c-sumo

5b

11a A+

12a A+

15b A+

16b A+

17b A+

19b A+

20b A+

21a,21b,21d A+

22b A+

23a,23b A+

23a-tev

24a K+

25b K+

26b K+

27b K+

28a,28b,28c K+

28a-sumo

28a-p53

29a K+

30a ,30c K+

31b A+

32a A+

32a pelB A+

35b K+

39b K+

40b K+

41a,41b k+

42a,42b K+

43b A+ 43.1a A+

44a A+

48b K+

49b K+ 50b K+ pETduet-1 pACYCduet-1 pCDFduet-1 pRSFduet-1 pColAduet-1 pET His pET DsbA pET GST pET Trx pQE载体PQE2 pQE9 pQE30,31,32, PQE30UA pQE30Laclq pQE40L pQE60 pQE70 pQE80L

其他原核表达载体